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KMID : 0385020030190040276
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Korean Journal of Laboratory Animal Science
2003 Volume.19 No. 4 p.276 ~ p.280
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Study on the Bcl-2 Expression in Fetal Kidneys Using a Tissue-array Technique
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Yoo Young-Bok
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Abstract
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Apoptosis is a genetically programmed cell death that is required for morphogenesis during embryogenic development and for tissue homeostasis in adult organisms. Although apoptosis is important in the development, expression of apoptosis-related genes has been studied mostly in the cancers and neurodegenerative diseases. We intended to obtain the basic data in order to understand the role of the apoptosis-related genes including bcl-2 in the apoptosis in the human development. Immunohiostochemistry for Bcl-2 was performed using Korean fetal kidney tissues in the 12~40 weeks of the human development. Our results showed that Bcl-2 expression was high in early stages of human development in the kidney. As differentiation grew, expression of Bcl-2 decreased and had the tendency of localizing in the tubular epitheliums, Bowman’s epithelium, We suggested that in the early stages when differentiation didn’t occur cell death was suppressed, in the late stages when differentiation was achieving cell death increased to remove the innecessary portions of the organs to protect the specific cells of the organs having functions. For the efficiency of the experiment, a high-throughput technique, a tissue-array method was applied which contributed to save time, money and labor without performance errors. Tissue-array technique will be useful to fasten the developmental studies.
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KEYWORD
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Fetal kidney, Apoptosis, Development, Bcl-2, Tissue-array
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